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Bio-intelligent manufacturing comprehensive innovation center

The Luculent Group's Comprehensive Innovation Center for Synthetic Biology Intelligent Manufacturing is composed of molecular biology laboratory, fermentation laboratory, testing laboratory, sterile laboratory, etc., including microfluidic high-throughput screening platform, bioinformatics + AI technology platform, molecular biology (genetic engineering) experimental platform, fermentation and detection platform, etc., which can carry out high-quality strain domestication and screening, strain creation, high-density fermentation, high-purity separation and purification, and full-project testing and identification.

Microfluidic high-throughput screening platform

The data-driven genotype construction platform based on high-throughput flow imaging has the characteristics of high throughput, automation, and high sensitivity. By using CRISPR high-throughput genotype construction, droplet microfluidics, and ultra-high-throughput characterization testing technology, the relationship between phenotypic genes, metabolism and correlation of production strains was deeply explored from the whole genome, such as analyzing the phenotype, genes, metabolism, etc. of HMOs production strains, and then screening out strains with better expression performance and more stable strains to achieve the construction of efficient strains.

advantage

It is compatible with flow detection and analysis microfluidic chips and static co-culture microwell chips, which can realize functions such as high-throughput flow imaging and static scanning, brightfield + multi-channel fluorescence, coaxial near-coherent light instantaneous focusing, and support single-cell PCR assays.

Bioinformatics + AI technology platform

The combination of bioinformatics and A!technology plays a synergistic role in enzyme research and proteomics research, providing us with powerful tools and methods for in-depth understanding of the function and properties of enzymes and the simulation of protein spatial structure. The breakthrough of generative AI technology has fully released the value of biological big data, and the complete data closed loop built by "generation, feedback, and optimization" can break through the limits of human computing and bring biological computing into the AI era.

advantage

Upstream optimization is empowered by AI algorithms, which has comprehensive advantages such as reducing by-products, reducing impurities, reducing separation and purification pressure in back-end production, and saving raw material costs.

Molecular biology (genetic engineering) experimental platform

Through gene editing technology, the required functional DNA fragments are introduced into model engineered bacteria, such as Escherichia coli and Pichia pastoris, to change the genetic information of the strains at the genetic level, and regulate their production capacity and the diversity of target products.

advantage

The main research objects are microorganisms such as alkaloidensis euoxygen, Escherichia coli, hydrolytic acidifying bacteria, yeasts, lactic acid bacteria, spores, and photosynthetic bacteria. Among them, Escherichia coli produces human milk oligosaccharides (HMOs), which provide nutrients closer to breast milk for infant milk powder and care for life and health.

Fermentation and testing platform

It is composed of fermentation laboratory and testing laboratory, equipped with fermentation tanks of different volumes of 5L-50L-100L-1000L, and the fermentation process is scaled up from 0-1-10 to establish the basic conditions for industrial production. The testing laboratory is equipped with a variety of precision testing instruments and develops a variety of detection methods to complete the detection and separation of target product concentration, impurities and isomer components, and realize the optimization of strain metabolism path, high-density fermentation, high-purity separation and purification, product quality control and other purposes.

advantage

The purity of the target products human milk oligosaccharides tetrasaccharides and neotetrasaccharides is > 92%, which is more than 20% higher than that produced by well-known foreign enterprises. The 100L-1000L fermentation tank can meet the sample mass production of some other high value-added biological products, and provide conditions for the industrialization of incubation of other biological products.